Bedoukian     Laser Vibrometer

Animal Taxa
Plant Taxa
Floral Compounds
Semiochemical Detail
Semiochemicals & Taxa
Invasive spp.


Email to a Friend
Kindly Donate for The Pherobase

« Previous AbstractMovements of native C505 during channel gating in CNGA1 channels    Next AbstractThe adenosine A2A antagonist MSX-3 reverses the effects of the dopamine antagonist haloperidol on effort-related decision making in a T-maze cost/benefit procedure »

Anal Bioanal Chem

Title:A MIP-based flow-through fluoroimmunosensor as an alternative to immunosensors for the determination of digoxin in serum samples
Author(s):Paniagua Gonzalez, G. Fernandez Hernando, P. Durand Alegria, J. S.
Address:Departamento de Ciencias Analiticas, Facultad de Ciencias, Universidad Nacional de Educacion a Distancia, 28040, Madrid, Spain.
Year:2009 Jun
Journal Title:Anal Bioanal Chem
Page Number:963-70
ISSN/ISBN:1618-2650 (Electronic)
Abstract:This work reports a comparative study of two automated flow-through fluorosensors for the determination of digoxin in serum samples: an immunosensor with an anti-digoxin polyclonal antibody as the reactive phase permanently immobilised on controlled-pore glass and a sensor with a selective reaction system based on a methacrylic molecularly imprinted polymer (MIP) synthesised by bulk polymerisation. The variables affecting the sensitivity and dynamic range of the sensors (e.g. the carrier and elution solutions, flow rates, pH and reagent concentrations) were optimized, and the binding characteristics of their reactive phases were compared in a competitive fluorescent assay. Digoxin was reproducibly determined by both sensors at the milligram per litre level (detection limit = 1.20 x 10(-3) mg L(-1) and RSD = 4-7% for the immunosensor; detection limit = 1.7 x 10(-5) mg L(-1) and RSD = 1-2% for the MIP sensor). No cross-reactivity with digoxin-related compounds was seen for either sensor at a digoxin/interferent ratio of 1:100. The lifetime of the immunosensor was about 50 immunoassays; its shelf life, when unused, is about 3 months. The lifetime of the MIP sensor was over 18 months. Both sensors were used to determine the digoxin concentration of human serum samples with satisfactory results.

Back to top
Citation: El-Sayed AM. The Pherobase: Database of Pheromones and Semiochemicals. <>.
The Pherobase - Extensive Database of Pheromones and Semiochemicals. Ashraf M. El-Sayed.